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CD46 monoclonal antibody (AT2G9)



ENZ-ABS722-0100	100 µl	433.00 USD
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Product Details

Alternative Name:	Membrane cofactor protein isoform 14, TLX, Trophoblast leukocyte common antigen

Clone:	AT2G9

Host:	Mouse

Isotype:	IgG2aκ

Immunogen:	Recombinant human CD46 (aa 35-313) purified from E. coli.

UniProt ID:	P15529

Source:	Purified from ascites.

Species reactivity:	Human

Applications:	ELISA, Flow Cytometry, ICC, IF, IHC, WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Protein A affinity purified

Formulation:	Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is implicated in the development and/or progression of selected cancer types.

Regulatory Status:	RUO - Research Use Only

Flow cytometry analysis of CD46 in PBMC cells. The cell was stained with ENZ-ABS722 at 2-5µg for 1x10⁶cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

ICC/IF analysis of CD46 in HeLa cells. The cell was stained with ENZ-ABS722 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Paraffin embedded sections of human colorectal cancer tissue were incubated with anti-human CD46 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

The cell lysates of Ramos, HeLa and A549 (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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