Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

CD73 monoclonal antibody (AT89E9)

ENZ-ABS719-0100 100 µl 445.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:Ecto-5'-nucleotidase, 5'-NT
Immunogen:Recombinant human CD73 (aa 27-252) purified from E. coli.
UniProt ID:P21589
Source:Purified from ascites.
Species reactivity:Human
Applications:ELISA, Flow Cytometry, ICC, IF, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein G affinity purified
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates enabling the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.
Regulatory Status:RUO - Research Use Only
CD73 monoclonal antibody (AT89E9) FACS
Flow cytometry analysis of CD73 in A431 cells. The cell was stained with ENZ-ABS719 at 2-5µg for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
CD73 monoclonal antibody (AT89E9) IF
ICC/IF analysis of CD73 in HeLa cells. The cell was stained with ENZ-ABS719 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
CD73 monoclonal antibody (AT89E9) WB
The cell lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HepG2 cell lysate. Lane 2: WiDr cell lysate. Lane 3: A431 cell lysate.
Please mouse over
CD73 monoclonal antibody (AT89E9) FACS CD73 monoclonal antibody (AT89E9) IF CD73 monoclonal antibody (AT89E9) WB

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
5'-nucleotidase Monoclonal antibody
By catalog section:


Technical Service
Customer Service