Alternative Name: | ζ-chain associated protein kinase |
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Clone: | 11H40 |
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Host: | Mouse |
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Isotype: | IgG1κ |
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Immunogen: | Recombinant full length human Zap-70. |
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UniProt ID: | P43403 |
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Source: | Purified from hybridoma tissue culture supernatant. |
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Species reactivity: | Human, Rat
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Applications: | IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (1:50)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~70kDa by Western blot. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Zeta-associated protein 70 (Zap-70) is a cytoplasmic protein tyrosine required for T cell antigen receptor (TCR) signaling and development. ZAP-70 interacts with the TCR by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. |
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Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human tonsil tissue using Zap-70 mAb (11H40), (Prod. No. ENZ-ABS709), at a dilution of 1:50. Panel A: Isotype Control, Panel B and C: ENZ-ABS709, diluted to 20µg/mL.
Immunohistochemistry analysis of human tonsil tissue using Zap-70 mAb (11H40), (Prod. No. ENZ-ABS709), at a dilution of 1:50. Panel A: Isotype Control, Panel B and C: ENZ-ABS709, diluted to 20µg/mL.
Immunohistochemistry analysis of human tonsil tissue using Zap-70 mAb (11H40), (Prod. No. ENZ-ABS709), at a dilution of 1:50. Panel A: Isotype Control, Panel B and C: ENZ-ABS709, diluted to 20µg/mL.
Western Blot analysis of Zap-70 mAb (11H40) (Prod. No. ENZ-ABS709): Lane 1: MW Marker, Lane 2: Jurkat lysate, Lane 3: Rat Thymus lysate.