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Perforin (mouse) monoclonal antibody (CB5.4)

 
ENZ-ABS708-0100 100 µg 465.00 USD
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Product Details

Clone:CB5.4
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse perforin (aa 98-534).
 
UniProt ID:P10820
 
Source:Purified from hybridoma tissue culture supernatant.
 
Species reactivity:Mouse
 
Specificity:Recognizes an epitope in the region aa 402-534.
 
Applications:ICC, IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~66kDa and higher (depending on PTM) by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Regulatory Status:RUO - Research Use Only
 
Perforin (mouse) monoclonal antibody (CB5.4) Western blot
Western Blot analysis of Perforin (mouse) mAb (CB5.4) (Prod. No. ENZ-ABS708): Lane 1: MW Marker, Lane 2: CTLL-2 cell lysate, Lane 3: 3T3 cell lysate. Western Blot analysis of Perforin 1 expression in CTLL-2 cell lysate and absence of perforin in the mouse fibroblast cell line 3T3.
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Perforin (mouse) monoclonal antibody (CB5.4) Western blot

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