| Alternative Name: | CD276, |
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| Clone: | 2A7 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Extracellular domain of human B7-H3. |
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| UniProt ID: | Q5ZPR3 |
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| Species reactivity: | Human
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| Applications: | Flow Cytometry, ICC, IF, IHC (PS), WB
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | CD276 was initially identified as a member of the B7 family of proteins. CD276 mRNA is widely expressed, but its protein expression is usually rather low. CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response. CD276 also plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity. It is a possible therapeutic target for at least a subset of cancers. |
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| Regulatory Status: | RUO - Research Use Only |
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Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 µg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
Western blot analysis of B7-H3 in HEK293 cells using B7-H3 antibody at (A) 0.25 (B) 0.5 and (C) 1 µg/ml.
Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 µg/ml.
Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 µg/ml. Green: B7-H3 Antibody [2A7] Blue: DAPI staining
Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 µg/ml.
