| Alternative Name: | VSIR, V-Type immunoglobulin domain-containing suppressor of T-cell activation |
| |
| Clone: | 4C4 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Extracellular domain of human VISTA. |
| |
| UniProt ID: | Q9H7M9 |
| |
| Species reactivity: | Human
|
| |
| Applications: | Flow Cytometry, ICC, IF, IHC (PS), WB
|
| |
| Purity Detail: | Protein A affinity purified. |
| |
| Formulation: | Liquid. In PBS containing 0.02% sodium azide and 50% glycerol. |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Shipped on Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 µg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 µg/mL.
Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 µg/mL.
Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 µg/mL. Green: VISTA Antibody [4C4] Blue: DAPI staining
Western blot analysis of VISTA in overexpressing HEK293 cells with VISTA antibody at (A) 0.25 (B) 0.5 and (C) 1 µg/ml
