| Alternative Name: | TNFSF14, CD258, HVEML |
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| Clone: | 7B9E12 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Extracellular domain of human LIGHT. |
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| UniProt ID: | O43557 |
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| Species reactivity: | Human
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| Applications: | Flow Cytometry, ICC, IF, IHC (PS), WB
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LTβR). While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells, LIGHT binding to LTβR is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction. LIGHT may become a potent tool in anti-cancer treatment. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 µg/ml
Immunohistochemistry of LIGHT in human lymphoma tissue with LIGHT antibody at 5 µg/mL.
Immunofluorescence of LIGHT in transfected HEK293 cells with LIGHT antibody at 2 µg/mL. Green: LIGHT Antibody [7B9E12] Blue: DAPI staining
Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 µg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.
Immunocytochemistry of LIGHT in transfected HEK293 cells with LIGHT antibody at 1 µg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 µg/mL.
