Programmed cell death 1 ligand 1, CD274, B7-H1, Programmed death ligand 1
Host:
Rabbit
Isotype:
IgG
Immunogen:
Recombinant PD-L1.
UniProt ID:
Q9NZQ7
Species reactivity:
Human
Applications:
IHC, WB
Recommended Dilutions/Conditions:
Immunohistochemistry (1:50-1:100)
Optimal conditions must be determined individually for each application.
Application Notes:
For IHC applications: Dilute the concentrate as appropriate in antibody diluent, and then apply 150 µL to the slide, as indicated in the user's IHC protocol.
Purity Detail:
Affinity-purified.
Formulation:
Liquid. In Tris buffer, pH 7.5, containing 1% BSA and <0.1% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Scientific Background:
PD-L1, also known as B7-H1 and CD274, is a 290 amino acid transmembrane glycoprotein in the B7 family of immune regulatory molecules. It has an extracellular domain that can interact with PD-1, which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. The PD1/PD-L1 interaction ensures that activation of the immune system occurs at the appropriate time. This will minimize the possibility of chronic autoimmune inflammation. PD-L1 is known to inhibit proliferation of T cells via apoptosis. It also plays an important role in arresting cell-cycle progression. This makes PD-L1 an attractive therapeutic target for cancer and immunologic diseases.
Regulatory Status:
RUO - Research Use Only
IHC using anti-PD-L1 (Prod. No. ENZ-ABS693) on lung adenocarcinoma tissue.
Formalin-fixed paraffin-embedded tonsil tissue stained using Enzo’s PD-L1 antibody (ENZ-ABS693). IHC staining was performed on an automated Leica BOND III.
Formalin-fixed paraffin-embedded lung tissue stained using Enzo’s PD-L1 antibody (ENZ-ABS693). IHC staining was performed on an automated Leica BOND III.
