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LAG-3 (human) recombinant monoclonal antibody (L4-PL33) (preservative free)

 
ENZ-ABS677PF-0100 100 µg 412.00 USD
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Product Specification

Alternative Name:Lymphocyte activation gene-3, FDC protein, CD223
 
Clone:L4-PL33
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).
 
UniProt ID:P18627
 
Source:Purified from tissue culture supernatant.
 
Species reactivity:Human
 
Specificity:Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of LAG-3.
 
Applications:ELISA, Flow Cytometry, ICC, IHC, IHC (FS), IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Immunohistochemistry (frozen sections, 1:150, FFPE sections, 1:150)
Western Blot (1-5 µg/ml)
Immunoprecipitation (10 µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 10% glycerol.
 
Use/Stability:Stable for at least 6 months after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.
 
Regulatory Status:RUO - Research Use Only
 
ENZ-ABS677 Flow
Flow cytometry analysis of 0.5x10^6 THP-1 cells stained using LAG-3 mAb (L4-PL33) at a concentration of 5μg/ml, followed by Alexa Fluor® 488 conjugated anti-mouse secondary antibody.
ENZ-ABS677-IHC
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tonsil tissue stained with LAG-3 (human) recombinant mAb (L4-PL33) at 6μg/ml.
ENZ-ABS677-WB-1
Western blot analysis of LAG-3 mAb (L4-PL33) (Prod. No. ENZ-ABS677): Lane 1: MW marker, Lane 2: LAG-3 (human):Fc (human), (recombinant) (Prod. No. ALX-522-078).
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ENZ-ABS677 Flow ENZ-ABS677-IHC ENZ-ABS677-WB-1

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