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WT1 Recombinant monoclonal antibody (WT1/1434R)

Monoclonal antibody to WT1 for use with IHC applications
 
ENZ-ABS363-0100 100 µg 466.00 USD
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Product Specification

Alternative Name:Wilms Tumor 1
 
Clone:WT1/1434R
 
Host:Rabbit
 
Isotype:IgGκ
 
Immunogen:Recombinant full length human WT1.
 
UniProt ID:P19544
 
Species reactivity:Human
 
Applications:Flow Cytometry, IF, IHC (PS)
 
Recommended Dilutions/Conditions:Flow Cytometry (0.5-1 µg/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 µg/mL)
Immunohistochemistry (formalin-fixed paraffin sections) (1:100-1:1000 or 0.5-1 µg/mL for 30 min at RT)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:The WT1 recombinant monoclonal antibody can be used to qualitatively identify the presence of WT1 protein in FFPE tissue sections using IHC test methods.
For IHC applications: Dilute the concentrate as appropriate in antibody diluent, and then apply 150 µL to the slide, as indicated in the user's IHC protocol.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
 
Regulatory Status:RUO - Research Use Only
 
WT1 Recombinant monoclonal antibody (WT1/1434R) IHC
Formalin-fixed paraffin-embedded wilms tumor 1 tissue stained using Enzo’s WT1 Recombinant monoclonal antibody (Prod. No. ENZ-ABS363). IHC staining was performed on an automated Leica BOND III.
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WT1 Recombinant monoclonal antibody (WT1/1434R) IHC

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