Alternative Name: | SSEA-1, Lewis X |
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Clone: | FUT4/1478R |
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Host: | Rabbit |
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Isotype: | IgGκ |
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Immunogen: | Purified human neutrophils. |
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Species reactivity: | Human, Mouse, Rat
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Applications: | Flow Cytometry, IF, IHC (PS)
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Recommended Dilutions/Conditions: | Flow Cytometry (2-4 µg/106 cells in 0.1 mL) Immunofluorescence (2-4 µg/mL) Immunohistochemistry (formalin-fixed paraffin sections) (5-10 µg/mL for 30 min at RT) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide. |
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Shipping: | Blue Ice |
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Long Term Storage: | +4°C |
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Scientific Background: | CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. |
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Regulatory Status: | RUO - Research Use Only |
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