| ENZ-ABS331-0100 | 100 µg | 554.00 USD |  |
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| Clone: | KRTH/1576R |
| Host: | Rabbit |
| Isotype: | IgGκ |
| Immunogen: | Recombinant full length human KRT76. |
| UniProt ID: | Q01546 |
| Species reactivity: | Human |
| Specificity: | This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8) |
| Applications: | Flow Cytometry, IF, IHC (PS), WB |
| Recommended Dilutions/Conditions: | Flow Cytometry (0.5-1 µg/106 cells in 0.1 mL) Immunofluorescence (1-2 µg/mL) Western blot (0.5-1 µg/mL) Immunohistochemistry (formalin-fixed paraffin sections) (0.25-0.5 µg/mL for 30 min at RT) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | +4°C |
| Scientific Background: | Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. |
| Regulatory Status: | RUO - Research Use Only |
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