| Clone: | r1415-1 |
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| Host: | Mouse |
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| Isotype: | lgG2aκ |
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| Immunogen: | Nuclei of human leukemia biopsy cells. |
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| UniProt ID: | P07305 |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | Flow Cytometry, IF, IHC (PS)
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| Recommended Dilutions/Conditions: | Flow Cytometry (1-2 µg/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 µg/mL)
Immunohistochemistry (formalin-fixed paraffin sections) (1-2 µg/mL for 30 min at RT)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
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| Regulatory Status: | RUO - Research Use Only |
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