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N6-methyladenosine monoclonal antibody (17-3-4-1)

Used to identify and purify methylated RNA and ssDNA.
 
ENZ-ABS301-0100 100 µg 325.00 USD
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Product Specification

Alternative Name:m6A
 
Clone:17-3-4-1
 
Host:Mouse
 
Isotype:IgG1λ
 
Immunogen:Hapten N6-methyladenosine-5’-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A).
 
Source:Purified from hybridoma tissue culture supernatant.
 
Species reactivity:Species independent
 
Specificity:Recognizes RNA or single stranded DNA that is methylated on the adenosine base at the nitrogen-6 position.
 
Applications:IP
Dot Blot
 
Recommended Dilutions/Conditions:Dot Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
 
Regulatory Status:RUO - Research Use Only
 
N6-methyladenosine monoclonal antibody (17-3-4-1) Immunoprecipitation
Figure 3. N6-methyladenosine, mAb (17-3-4-1) (prod. no. ENZ-ABS301) can be used to purify synthetic methylated transcripts. Radiolabeled control non-methylated (350 nt) and methylated (250 nt) input transcripts can be separated using IP.
N6-methyladenosine monoclonal antibody (17-3-4-1) Immunoprecipitation
Figure 2. North Western blot using synthetic methylated and non-methylated RNA detected with N6-methyladenosine, mAb (17-3-4-1).
N6-methyladenosine monoclonal antibody (17-3-4-1) Western blot
Figure 1. Western blot analysis showing context recognition. (A) N6-methyladenosine, mAb (17-3-4-1) (Prod No. ENZ-ABS301). (B) Commercial rabbit polyclonal antibody.
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N6-methyladenosine monoclonal antibody (17-3-4-1) Immunoprecipitation N6-methyladenosine monoclonal antibody (17-3-4-1) Immunoprecipitation N6-methyladenosine monoclonal antibody (17-3-4-1) Western blot

Product Literature References

Development and validation of monoclonal antibodies against N6-methyladenosine for the detection of RNA modifications: S. Matsuzawa, et al.; PLoS One 14, e0223197 (2019), Abstract; Full Text

General Literature References

An Adenine Code for DNA: A Second Life for N6-Methyladenine: H. Heyn, et al.; Cell 161, 710 (2015), (Review), Abstract;
DNA Methylation on N6-Adenine in C. elegans: E.L. Greer, et al.; Cell 161, 868 (2015), Abstract;
N6-Methyladenine DNA Modification in Drosophila: G. Zhang, et al.; Cell 161, 893 (2015), Abstract;
N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas: Y Fu, et al.; Cell 161, 879 (2015), Abstract;

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