Product Specification
| Alternative Name: | m6A |
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| Clone: | 17-3-4-1 |
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| Host: | Mouse |
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| Isotype: | IgG1λ |
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| Immunogen: | Hapten N6-methyladenosine-5’-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A). |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes RNA or single stranded DNA that is methylated on the adenosine base at the nitrogen-6 position. |
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| Applications: | IP
Dot Blot
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| Recommended Dilutions/Conditions: | Dot Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Scientific Background: | N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 3. N6-methyladenosine, mAb (17-3-4-1) (prod. no. ENZ-ABS301) can be used to purify synthetic methylated transcripts. Radiolabeled control non-methylated (350 nt) and methylated (250 nt) input transcripts can be separated using IP.
Figure 2. North Western blot using synthetic methylated and non-methylated RNA detected with N6-methyladenosine, mAb (17-3-4-1).
Figure 1. Western blot analysis showing context recognition. (A) N6-methyladenosine, mAb (17-3-4-1) (Prod No. ENZ-ABS301). (B) Commercial rabbit polyclonal antibody.
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Product Literature References
Development and validation of monoclonal antibodies against N6-methyladenosine for the detection of RNA modifications: S. Matsuzawa, et al.; PLoS One
14, e0223197 (2019),
Abstract;
Full Text
General Literature References
An Adenine Code for DNA: A Second Life for N6-Methyladenine: H. Heyn, et al.; Cell
161, 710 (2015), (Review),
Abstract;
DNA Methylation on N6-Adenine in C. elegans: E.L. Greer, et al.; Cell
161, 868 (2015),
Abstract;
N6-Methyladenine DNA Modification in Drosophila: G. Zhang, et al.; Cell
161, 893 (2015),
Abstract;
N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas: Y Fu, et al.; Cell
161, 879 (2015),
Abstract;
