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ERGIC-53 monoclonal antibody (OTI1A8)

ENZ-ABS300-0100 100 µl 597.00 USD
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Product Details

Alternative Name:ER-Golgi intermediate compartment 53kDa protein, LMAN1
Immunogen:Full length recombinant human ERGIC-53.
UniProt ID:P49257
Species reactivity:Human, Rat
Dog, Monkey
Applications:Flow Cytometry, IF, IHC, WB
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (1:150)
Western Blot (1:200-1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Affinity purified.
Formulation:Liquid. In PBS, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 is a mannose-specific membrane lectin operating as a cargo receptor for the transport of glycoproteins from the ER to the ERGIC. ERGIC-53 can also be used for studying aspects of protein trafficking in the secretory pathway.
Regulatory Status:RUO - Research Use Only
ERGIC-53 monoclonal antibody (OTI1A8) Flow Cytometry
Flow cytometric analysis of Jurkat cells, using ERGIC-53 monoclonal antibody (OTI1A8) (red), compared to a nonspecific negative control antibody (blue).
ERGIC-53 monoclonal antibody (OTI1A8) Immunofluorescence
ERGIC-53 monoclonal antibody (OTI1A8) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERGIC-53.
ERGIC-53 monoclonal antibody (OTI1A8) Immunohistochemistry
Immunohistochemistry staining of paraffin-embedded human prostate tissue within the normal limits using ERGIC-53 monoclonal antibody (OTI1A8).
ERGIC-53 monoclonal antibody (OTI1A8) Immunohistochemistry
Immunohistochemistry staining of paraffin-embedded carcinoma of human prostate tissue using ERGIC-53 monoclonal antibody (OTI1A8).
ERGIC-53 monoclonal antibody (OTI1A8) Western blot
Western blot analysis of extracts (35µg) from 9 different cell lines by using ERGIC-53 monoclonal antibody (OTI1A8).
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ERGIC-53 monoclonal antibody (OTI1A8) Flow Cytometry ERGIC-53 monoclonal antibody (OTI1A8) Immunofluorescence ERGIC-53 monoclonal antibody (OTI1A8) Immunohistochemistry ERGIC-53 monoclonal antibody (OTI1A8) Immunohistochemistry ERGIC-53 monoclonal antibody (OTI1A8) Western blot

Product Literature References

African swine fever virus transmembrane protein pEP84R guides core assembly: A. Alejo, et al.; PLoS Pathog. 19, e1011136 (2023), Abstract;
C11orf94/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly: W. Contreras, et al.; Sci. Adv. 8, eabo6049 (2022), Abstract;
Expression of the Heterotrimeric GP2/GP3/GP4 Spike of an Arterivirus in Mammalian Cells: A.K. Matczuk, et al.; Viruses 14, 749 (2022), Abstract;
The Envelope protein of SARS-CoV-2 increases intra-Golgi pH and forms a cation channel that is regulated by pH: D.C. Garcia, et al.; J. Physiol. 599, 2851 (2021), Abstract;
The P429L loss of function mutation of the human glycine transporter 2 associated with hyperekplexia: A. Kitzenmaier, et al.; Eur. J. Neurosci. 50, 3906 (2019), Abstract; Full Text
Cutis laxa, exocrine pancreatic insufficiency and altered cellular metabolomics as additional symptoms in a new patient with ATP6AP1-CDG: B. Dimitrov, et al.; Mol. Genet. Metab. 123, 364 (2018), Abstract;
Surf4 (Erv29p) binds amino-terminal tripeptide motifs of soluble cargo proteins with different affinities, enabling prioritization of their exit from the endoplasmic reticulum: Y. Yin, et al.; PLoS Biol. 16, e2005140 (2018), Abstract; Full Text
Assembly and release of infectious hepatitis C virus involving unusual oganization of the secretory pathway: M. Triyatni, et al.; World J. Hepatol. 8, 796 (2016), Application(s): Western blot, human serum, Abstract; Full Text
Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus: C.M. Dorobantu, et al. ; PLoS One 11, e1005185 (2015), Application(s): Immunofluorescence microscopy, Abstract; Full Text

General Literature References

Coronavirus envelope (E) protein remains at the site of assembly: P. Venkatagopalan, et al.; Virology 478C, 75 (2015), Abstract;
The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway: P. Roboti, et al.; J. Cell. Sci. 128, 1595 (2015), Abstract; Full Text
Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex: R.K. Morikawa, et al.; J. Biol. Chem. 284, 26620 (2009), Abstract;
Deletion of 3 residues from the C-terminus of MCFD2 affects binding to ERGIC-53 and causes combined factor V and factor VIII deficiency: B. Nyfeler, et al.; Blood 111, 1299 (2008), Abstract;
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell 19, 1976 (2008), Abstract;
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS 102, 6350 (2005), Abstract; Full Text
pH-induced conversion of the transport lectin ERGIC-53 triggers glycoprotein release: C. Appenzeller-Herzog, et al.; J. Biol. Chem. 279, 12943 (2004), Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004), Abstract; Full Text
ERGIC-53 and traffic in the secretory pathway: H.P. Hauri, et al.; J. Cell Sci. 113, 587 (2000), Abstract; Full Text
The recycling of ERGIC-53 in the early secretory pathway. ERGIC-53 carries a cytosolic endoplasmic reticulum-exit determinant interacting with COPII: F. Kappeler, et al.; J. Biol. Chem. 272, 31801 (1997), Abstract; Full Text
ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins: C. Itin, et al.; Mol. Biol. Cell 7, 483 (1996), Abstract; Full Text
Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol. 131, 57 (1995), Abstract; Full Text
A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53: F. Kappeler, et al.; J. Biol. Chem. 269, 6279 (1994), Abstract; Full Text
The isolated ER-Golgi intermediate compartment exhibits properties that are different from ER and cis-Golgi: A. Schweizer, et al.; J. Cell Biol. 113, 45 (1991), Abstract; Full Text
Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus: A. Schweizer, et al.; J. Cell Biol. 107, 1643 (1988), Abstract; Full Text

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