| ENZ-ABS271-0100 | 100 µg | 340.00 USD |  |
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| Alternative Name: | Oct3, OTF3, OTF4, POU5F1 |
| Clone: | 9B7 |
| Host: | Mouse |
| Isotype: | IgG |
| Immunogen: | Full-length recombinant human Oct4 produced in E. coli |
| UniProt ID: | Q01860 |
| Species reactivity: | Human |
| Specificity: | Recognizes human Oct4 in embryonic carcinomic samples, but not in adult samples (e.g. HeLa cells). |
| Applications: | Flow Cytometry, ICC, IF, WB |
| Recommended Dilutions/Conditions: | The optimal working dilution should be determined for each specific assay condition. Flow Cytometry (Flow): 3-5 µg/1x106 cells Immunocytochemistry (ICC/IF): 1-2 µg/mL Western Blot (WB) 1:500-1:5000 |
| Application Notes: | Detects a band of ~40kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In PBS containing 1mg/ml BSA, 30% glycerol, and 0.05% sodium azide. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | Oct4 is a transcription factor which plays a key role in embryonic development, especially during early embryogenesis. Oct4 is one of the four Yamaka factors, necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. |
| Regulatory Status: | RUO - Research Use Only |
