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Digoxigenin polyclonal antibody

Species independent all-purpose immuno-tag.
ENZ-ABS266-0100 100 µg 108.00 USD
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Product Details

Source:Purified from sheep serum.
Species reactivity:Species independent
Specificity:Recognizes Digoxin and Digoxigenin.
Applications:ELISA, WB
ISH (in situ hybridization), TUNEL
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. DIG is the steroid component of digoxin, a cardiac glycoside, and can be conjugated onto a variety of biomolecules (proteins, nucleic acids) to be detected in further assays (e.g. ELISA). These antibodies can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection.

DIG has an advantage over biotin due to the fact that biotin is an endogenous molecule of living cells and plays a key role in many reactions, which can sometimes lead to false positive results. Because the blossoms and leaves of Digitalis plants are the only natural sources of digoxigenin, binding of the anti-digoxigenin antibody in other biological material is not a consideration.
Technical Info/Product Notes:This product is unconjugated but if you desire a specific conjugation please contact technical support for lead time and pricing. No minimum quantity required.
Regulatory Status:RUO - Research Use Only
Digoxigenin polyclonal antibody TUNEL
Apoptotic nuclei in an ischemic model of brain injury visualized by TUNEL using digoxigenin polyclonal antibody (Prod. No. ENZ-ABS302) and horseradish peroxidase.
Digoxigenin polyclonal antibody Western blot
Western blot analysis of Digoxigenin, pAb (Prod. No. ENZ-ABS302): Lane 1: MW marker, Lane 2: LTC4, mAb (Digoxingenin conjugate), Lane 3: IL-8, mAb (Digoxigenin conjugate).
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Digoxigenin polyclonal antibody TUNEL Digoxigenin polyclonal antibody Western blot

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