| ENZ-ABS259-0100 | 100 µg | 593.00 USD |  |
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| Alternative Name: | CARD adapter inducing interferon-β, IPS-1, Interferon-β promoter stimulator protein 1, Mitochondrial antiviral signalling protein, VISA, Virus-induced signalling adapter |
| Clone: | 58N3B6 |
| Host: | Mouse |
| Isotype: | IgG2bκ |
| Immunogen: | Human MAVS (IPS-1) |
| UniProt ID: | Q7Z434 |
| Gene/Protein Identifier: | 57506 (Entrez GeneID) |
| Species reactivity: | Human |
| Applications: | IHC (PS), WB |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1:10-1:2,000) (paraffin sections 3-5µg/ml) Western Blot (2-5µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Protein G purified. |
| Formulation: | Liquid. In 0.2ml PBS containing 0.05% BSA and 0.05% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Shipping: | Shipped on Blue Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Scientific Background: | RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production. |
| Regulatory Status: | RUO - Research Use Only |
