Western blot of anti-Keratin 1 antibody (Poly19052). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 μg/ml for 24 hours at 4°C, followed by incubation with a goat antirabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection.
