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WCM (Wash Medium)

ENZ-70002-0200 200 ml 57.00 USD
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For washing of spleen cells and myeloma fusion partner to remove all serum prior to the fusion process and to wash cells during fluorescent labeling.

Product Specification

Alternative Name:Hybridoma Dish™ Kit WCM
Formulation:Iscove's Modified Dulbecco's Medium based medium containing antibiotics and antimycotics
Application Notes:Uses:
Hybridoma generation
Cell line development
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Handling:Refrigerate or freeze upon arrival.
Regulatory Status:RUO - Research Use Only

Product Literature References

Engineered cell surface expression of membrane immunoglobulin as a means to identify monoclonal antibody-secreting hybridomas: P.W. Price, et al.; J. Immunol. Methods 343, 28 (2009), Application(s): Hyridoma screening, Abstract; Full Text

General Literature References

Antibody production by hybridomas: J.W. Goding; J. Immunol. Methods 39, 285 (1980), Abstract;
Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies: J. Sharon, et al.; PNAS 76, 1420 (1979), Abstract; Full Text
Continuous culture of fused cells secreting antibody of predefined specificity: G. Köhler & C. Milstein; Nature 256, 495 (1975), Abstract; Full Text

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