Mouse Dkk-1 amino acids 33-272, as well as a TEV recognition site and 6 histidines at the C terminus.
MW:
~40kDa (SDS-PAGE)
Source:
Produced in insect cells. Produced in a baculovirus expression system.
UniProt ID:
O54908
Quantity:
5µg
Formulation:
Lyophilized from a solution containing 1X PBS, 1% CHAPS, and 0.1% BSA.
Purity:
≥80% (SDS-PAGE)
Endotoxin Content:
<1.0EU/µg (LAL method)
Biological Activity:
Determined measuring the ability to inhibit the induction of the LEADING LIGHT® Wnt Reporter Cell Line (Prod. No. ENZ-61002) induced for at least 6 hours with 200 ng/ml Wnt3a (Prod. No. ENZ-60001). The EC50 of this inhibition is 45-55 ng/ml.
Reconstitution:
Reconstitute by adding 1.5ml water to make a 3.3µg/ml solution.
Shipping:
Shipped on Dry Ice
Short Term Storage:
-80°C
Long Term Storage:
-80°C
Use/Stability:
Stable for at least 6 months after receipt when stored as supplied at -80°C.
Handling:
Avoid freeze/thaw cycles.
Scientific Background:
Dickkopf-related protein 1 (Dkk-1), which belongs to the dickkopf family, is a secreted protein with two cysteine rich domains and is involved in embryonic development and many human diseases. The regulation of Wnt signaling and Dkk1 inhibition has implications for bone formation and homeostatis. Dkk-1 can inhibit Wnt signaling by binding to its receptors LRP5/6 and kremen1/2 to form a complex, which can prevent Wnt from binding to LRP5/6. The predicted mouse Dkk1 protein is 272 amino acid residues with an N-terminal signal peptide. It shares 86% amino acid identity to human Dkk-1 protein.
