- Inhibits photobleaching of fluorescent dyes and fluorescent proteins
- Hardening formulation
Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.
Product Details
| Applications: | IF, IHC, Fluorescence microscopy, Fluorescent detection
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| Shipping: | Blue Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Scientific Background: | All fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the dye, the intensity and the time of illumination. Many products have been developed to prevent fading of fluorescent dyes. These products often work well for one or more dyes, but often cause problems, such as degradation of other dyes. Antifade mounting medium (hardset) works well with many stains including fluorescein and rhodamine. |
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| Technical Info/Product Notes: | The Antifade mounting medium (hardset) is recommended for use on fixed cells and tissues that have been stained with fluorescent stains. Cells can be viewed immediately, or if long term storage is desired, the reagent can be allowed to harden overnight. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies: D. Shen, et al.; Cell Biochem. Biophys.
60, 173 (2011),
Abstract;
Full Text
General Literature References
Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy.: A. Diaspro et al.; Appl. Opt.
41, 685 (2002),
Abstract;
Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy. : M. Ono et al.; J. Histochem. Cytochem.
49, 305 (2001),
Abstract;
Full Text
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