Nile red is a lipophilic probe that becomes intensely fluorescent in a hydrophobic environment, while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy, in cells such as adipocyes. Flow cytometry methods using a single staining with Nile red and double staining with Nile red and anti-CD3 monoclonal antibody have been employed to monitor peripheral leukocyte and lymphocyte phospholipidosis. Nile Red has also been used for rapid staining of proteins in SDS-polyacrylamide gel electrophoresis. Wavelength Maxima: Excitation 552nm, Emission 636nm
Product Details
| MW: | 318.4 |
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| CAS: | 7385-67-3 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A clinical flow cytometric biomarker strategy: validation of peripheral leukocyte phospholipidosis using Nile red: B.W. Halstead, et al.; J. Appl. Toxicol.
26, 169 (2006),
Abstract;
Detection of five nanograms of protein by two-minute nile red staining of unfixed SDS gels: F.J. Alba, et al; Biotechniques
21, 625 (1996),
Abstract;
