NBD-Cl is widely used to label peptides, proteins, drugs and other biomolecules. NBD-Cl is non-fluorescent, and generates green fluorescent adducts (emission maximum of 512 nm) upon reacting with aliphatic amines or thiol compounds. It reacts with amino groups such as aliphatic amines, amino acids, peptides, and proteins to form highly fluorescent compounds. The reagent has been used in a variety of spectrophotometric, spectrofluorometric and HPLC assays of pharmaceutically important analytes. Wavelength Maxima: Excitation 337nm
Product Details
| Alternative Name: | 4-Chloro-7-nitrobenzofurazan, 4-Chloro-7-nitro-2,1,3-benzoxadiazole |
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| MW: | 199.6 |
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| CAS: | 10199-89-0 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Spectrofluorimetric determination of fluoroquinolones in pharmaceutical preparations: S.T. Ulu; Spectrochim. Acta A Mol. Biomol. Spectrosc.
72, 138 (2009),
Abstract;
Spectrophotometric, spectrofluorometric and HPLC determination of desloratadine in dosage forms and human plasma: N. El-Enany, et al.; Chem. Pharm. Bull. (Tokyo)
55, 1662 (2007),
Abstract;
