Fluorescein isothiocyanate (FITC) is an amine-reactive fluorescein derivative that yields conjugates with superior stability. Dye synthesis generally results in two isomers that differ slightly in protein binding and may elute differently under chromatographic conditions, complicating analysis in certain situations. The 5-isomer of FITC is the most widely used isoform and has a multitude of applications beyond protein peptide and nucleotide labeling. For example, it is commonly employed as a donor for FRET (Fluorescence Resonance Energy Transfer) based assay systems. An acceptor molecule, such as TRITC, is recommended in such FRET applications. Wavelength Maxima: Excitation 494nm, Emission 520nm
Product Details
| Alternative Name: | 5-Fluorescein Isothiocyanate |
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| Formula: | C21H11NO5S |
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| MW: | 389.4 |
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| CAS: | 3326-32-7 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Polymeric nanoparticles for protein kinase activity: J.H. Kim, et al.; Chem. Commun. (Camb.)
2007, 1346 (2007),
Abstract;
Proximity relationships and structural dynamics of the phalloidin binding site of actin filaments in solution and on single actin filaments on heavy meromyosin: M. Heidecker, et al.; Biochemistry
34, 11017 (1995),
Abstract;
