Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). Acridine Orange is also commonly used to non-specifically stain acidic organelles, such as lysosomes The dye is often used in epifluorescence microscopy. Wavelength Maxima: Excitation 502nm, Emission 525nm
Product Details
| Alternative Name: | N,N,N’,N’-Tetramethyl-3,6-acridinediamine . HCl |
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| Formula: | C17H19N3 . HCl |
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| MW: | 301.8 |
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| CAS: | 65-61-2 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Orange powder. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Acridine orange differential staining of total DNA and RNA in normal and galactosemic lens epithelial cells in culture using flow cytometry: K. Gonzalez, et al.; Curr. Eye Res.
14, 269 (1995),
Abstract;
Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations: F. Traganos & Z. Darzynkiewicz; Methods Cell Biol.
41, 185 (1994),
Abstract;
