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DAPI (ultra pure)

DNA dye
ENZ-52404 100 mg 740.00 USD
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DAPI is a fluorescent dye that binds nucleic acids and demonstrates a high affinity for DNA. It is used extensively in fluorescence microscopy, flow cytometry and microplate assays. DAPI is cell permeable and is readily used for detecting both live, dead and fixed cells. DAPI emits in the blue spectral range and is ideal for multiplexed assays inclusive of green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Aside from labeling cell nuclei, DAPI is also used for the detection of mycoplasma or viral DNA in cell cultures. Wavelength Maxima: Excitation 358nm, Emission 461nm

Product Details

Alternative Name:4’,6-Diamidino-2-phenylindole . dihydrochloride
Purity:≥95% (HPLC)
Solubility:Soluble in DMSO.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Stable for at least one year after receipt when stored as recommended.
Handling:Protect from light. Keep cool and dry.
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Regulatory Status:RUO - Research Use Only
ENZ-52404 structure
DAPI (ultra pure) Fig2
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ENZ-52404 structure DAPI (ultra pure) Fig2

Product Literature References

Fmr1-KO mice failure to detect object novelty associates with a post-test decrease of structural and synaptic plasticity upstream of the hippocampus: A. Borreca, et al.; Sci. Rep. 13, 755 (2023), Abstract;
Undercarboxylated, But Not Carboxylated, Osteocalcin Suppresses TNF-α-Induced Infammatory Signaling Pathway in Myoblasts: D. Park, et al.; J. Endocr. Soc. 6, bvac084 (2022), Abstract;
Light-induced osteogenic differentiation of BMSCs with graphene/TiO2 composite coating on Ti implant: X. Long, et al.; Colloids Surf. B Biointerfaces 207, 111996 (2021), Abstract;
Conidiobolus coronatus induces oxidative stress and autophagy response in Galleria mellonella larvae: M. Kazek, et al.; PLoS One 15, e0228407 (2020), Abstract; Full Text
GRP78-Mediated Signaling Contributes to Axonal Growth Resulting in Motor Function Recovery in Spinal Cord-Injured Mice: Y. Tanie, et al.; Front. Pharmacol. 11, 789 (2020), Abstract; Full Text
Effect of strontium doping on the biocompatibility of calcium phosphate-coated titanium substrates: T.T. Nguyen, et al.; J. Appl. Biomater. Funct. Mater. 17, 2280800019826517 (2019), Abstract; Full Text
Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells: X. Chen, et al.; Methods Mol. Biol. 1884, 43 (2019), Abstract; Full Text
Matrine promotes neural circuit remodeling to regulate motor function in a mouse model of chronic spinal cord injury: N. Tanabe, et al.; Neural Regen. Res. 14, 1961 (2019), Abstract;
Oxidative Damage in Human Periodontal Ligament Fibroblast (hPLF) after Methylmercury Exposure: L.S. Nogueira, et al.; Oxid. Med. Cell. Longev. 2019, 8470857 (2019), Abstract; Full Text
Transplantation of human islets without immunosuppression: B. Ludwig, et al.; PNAS 110, 19054 (2013), Application(s): Immunofluorescence on PPFE tissue sections, Abstract;
Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry--new prospects for plant research: J. Suda & P. Travnicek; Cytometry A 69, 273 (2006), Abstract;
DAPI fluorescence in nuclei isolated from tumors: A. Krishan & P.D. Dandekar; J. Histochem. Cytochem. 53, 1033 (2005), Abstract;

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