Replaces Prod. #: ALX-620-051
Hoechst 33258 is a fluorescent probe useful for detecting DNA by fluorescence microscopy and flow cytometry. Hoechst 33258 may be used on live or fixed cells and is also applicable for cell cycle analysis and monitoring DNA condensation by flow cytometry. Hoechst 33358 is less cell permeable that other derivatives, however this dye also allows for quantitative measurements when plotted in a standard emission-to-content curve. Wavelength Maxima: Excitation 352nm, Emission 461nm
Product Details
| Alternative Name: | 4-[5-(4-Methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-phenol . trihydrochloride |
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| Formula: | C25H24N6O . 3HCl |
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| MW: | 533.9 (trihydrochloride) |
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| CAS: | 23491-45-4 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Yellow solid. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Otoprotective Effects of Fucoidan Reduce Cisplatin-Induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: C.Y. Hsieh, et al.; Int. J. Mol. Sci.
24, 3561 (2023),
Abstract;
Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway - implications for Parkinson’s disease: W. Tang, et al.; Hum. Mol. Genet.
32, 1466 (2023),
Abstract;
Bisdemethoxycurcumin-mediated Attenuation of Apoptosis Prevents Gentamicin-induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: T.Y. Kang, et al.; In Vivo
36, 1095 (2022),
Abstract;
Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae: A new protocol: A.K. Wrońska, et al.; PLoS One
17, e0274120 (2022),
Abstract;
Nordihydroguaiaretic acid (NDGA) and α-mangostin inhibit the growth of Mycobacterium tuberculosis by inducing autophagy: S. Guzman-Beltran, et al.; Int. Immunopharmacol.
31, 149 (2016),
Application(s): Counter-staining,
Abstract;
Hormone-induced 14-3-3γ adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells: Y. Aghazadeh, et al.; J. Biol. Chem.
287, 15380 (2012),
Application(s): Nuclear staining of Adult mouse testis sections,
Abstract;
Full Text
DNA condensation monitoring after interaction with hoechst 33258 by atomic force microscopy and fluorescence spectroscopy: M. Saito, et al.; J. Biochem.
136, 813 (2004),
Abstract;
Cell cycle analysis using continuous bromodeoxyuridine labeling and Hoechst 33258-ethidium bromide bivariate flow cytometry: M. Poot, et al.; Methods Cell Biol.
33, 185 (1990),
Abstract;
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