Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Hoechst 33258 (ultra pure)

DNA dye
ENZ-52402 100 mg 167.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ALX-620-051

Hoechst 33258 is a fluorescent probe useful for detecting DNA by fluorescence microscopy and flow cytometry. Hoechst 33258 may be used on live or fixed cells and is also applicable for cell cycle analysis and monitoring DNA condensation by flow cytometry. Hoechst 33358 is less cell permeable that other derivatives, however this dye also allows for quantitative measurements when plotted in a standard emission-to-content curve. Wavelength Maxima: Excitation 352nm, Emission 461nm

Product Details

Alternative Name:4-[5-(4-Methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-phenol . trihydrochloride
Formula:C25H24N6O . 3HCl
MW:533.9 (trihydrochloride)
Purity:≥95% (HPLC)
Appearance:Yellow solid.
Solubility:Soluble in DMSO.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Stable for at least one year after receipt when stored as recommended.
Handling:Protect from light. Keep cool and dry.
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Regulatory Status:RUO - Research Use Only
Please mouse over

Product Literature References

Otoprotective Effects of Fucoidan Reduce Cisplatin-Induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: C.Y. Hsieh, et al.; Int. J. Mol. Sci. 24, 3561 (2023), Abstract;
Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway - implications for Parkinson’s disease: W. Tang, et al.; Hum. Mol. Genet. 32, 1466 (2023), Abstract;
Bisdemethoxycurcumin-mediated Attenuation of Apoptosis Prevents Gentamicin-induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: T.Y. Kang, et al.; In Vivo 36, 1095 (2022), Abstract;
Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae: A new protocol: A.K. Wrońska, et al.; PLoS One 17, e0274120 (2022), Abstract;
Nordihydroguaiaretic acid (NDGA) and α-mangostin inhibit the growth of Mycobacterium tuberculosis by inducing autophagy: S. Guzman-Beltran, et al.; Int. Immunopharmacol. 31, 149 (2016), Application(s): Counter-staining, Abstract;
Hormone-induced 14-3-3γ adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells: Y. Aghazadeh, et al.; J. Biol. Chem. 287, 15380 (2012), Application(s): Nuclear staining of Adult mouse testis sections, Abstract; Full Text
DNA condensation monitoring after interaction with hoechst 33258 by atomic force microscopy and fluorescence spectroscopy: M. Saito, et al.; J. Biochem. 136, 813 (2004), Abstract;
Cell cycle analysis using continuous bromodeoxyuridine labeling and Hoechst 33258-ethidium bromide bivariate flow cytometry: M. Poot, et al.; Methods Cell Biol. 33, 185 (1990), Abstract;

Related Products

Hoechst 33342 (ultra pure) 

Cell permeable DNA dye
23491-52-3, ≥95% (HPLC) | Print as PDF
ENZ-52401 100 mg 167.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Organelle Dye
Nucleus Dye
By catalog section:


Technical Service
Customer Service

Related Literature

Live Cell Analysis
Live Cell Analysis
Download as PDF

All new literature pieces