DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environmentsensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. DiIC12(3) is a lipophilic neuronal tracer that is commonly used for labeling neuronal projections as well as lipid bilayers in other cell types, particularly endothelial cells. DiIC12(3) exhibits low toxicity and minimally effects cell viability and is tolerable in culture for several days, making it suitable across a variety of applications. Wavelength Maxima: Excitation 549nm, Emission 565nm
Product Details
| Alternative Name: | 1,1’-Didodecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate |
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| Formula: | C47H73N2O4Cl |
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| MW: | 765.5 |
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| CAS: | 75664-01-6 |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | Dark red powder. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Importin 8 mediates m7G cap-sensitive nuclear import of the eukaryotic translation initiation factor eIF4E: L. Volpon, et al.; PNAS
113, 5263 (2016),
Application(s): Cell culture,
Abstract;
Full Text
Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI: Y. Li, et al.; Nat. Protoc.
3, 1703 (2008),
Abstract;
Multiphoton imaging of chick retinal development in relation to gap junctional communication: D.L. Becker, et al.; J. Physiol.
585, 711 (2007),
Abstract;
