DiBAC4(3) is a sensitive slow-response probe for measuring cellular membrane potential. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of response is much larger than fast-response probes. Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors. DiBAC4(3) has been employed in flow cytometry to monitor antibacterial activity of defensins. Wavelength Maxima: Excitation 493nm, Emission 516nm
Product Details
| Alternative Name: | bis-(1,3-Dibarbituric acid)-trimethine oxonol |
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| Formula: | C27H40N4O6 |
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| MW: | 516.6 |
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| CAS: | 70363-83-6 |
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| Purity: | ≥98% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Contribution of Particle-Induced Lysosomal Membrane Hyperpolarization to Lysosomal Membrane Permeabilization: T. Ziglari, et al.; Int. J. Mol. Sci.
22, 2277 (2021),
Abstract;
Full Text
Characterization of excitability and voltage-gated ion channels of neural progenitor cells in rat hippocampus: T. Li, et al.; J. Mol. Neurosci.
35, 289 (2008),
Abstract;
A flow cytometric assay to monitor antimicrobial activity of defensins and cationic tissue extracts: S. Nuding, et al.; J. Microbiol. Methods
65, 335 (2006),
Abstract;
