Lucigenin is a blue-green fluorescent chloride ion indicator that is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells. Lucigenin is a fluorescent indicator that is quenched via collision with chloride. The probe is suitable for detection of chloride concentrations in liposomes, reconstituted membranes or in extracellular medium. Lucigenin may also be used as a chemiluminescent probe for the detection of superoxide in biological systems. Wavelength Maxima: Excitation 455nm, Emission 505nm
Product Details
Alternative Name: | bis-N-Methylacridinium nitrate |
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Formula: | C28H22N4O6 |
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MW: | 510.5 |
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CAS: | 22103-92-0 |
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Purity: | ≥98% (HPLC) |
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Appearance: | Yellow solid. |
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Solubility: | Soluble in DMSO. |
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Shipping: | Ambient Temperature |
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Long Term Storage: | -20°C |
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Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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Handling: | Protect from light. Keep cool and dry. |
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Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes: M. Kervinen, et al.; Anal. Biochem.
324, 45 (2004),
Abstract;
Vacuolar chloride transport in Mesembryanthemum crystallinum L. measured using the fluorescent dye lucigenin: F. Wissing & J.A. Smith; J. Membr. Biol.
177, 199 (2000),
Abstract;