CFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. FDA and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular nonspecific esterases, yielding fluorescent probes. These probes accumulate exclusively in cells with intact cell membranes. CFDA does not stain dead cells. FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells. Wavelength Maxima: Excitation 494nm, Emission 521nm
Product Details
| Alternative Name: | 5(6)-Carboxyfluorescein diacetate (ultra pure) |
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| Formula: | C25H16O9 |
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| MW: | 460.4 |
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| CAS: | 124387-19-5 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester: B.J. Quah, et al.; Nat. Protoc.
2, 2049 (2007),
Abstract;
Carboxyfluorescein (CFSE) labelling of hepatocytes for short-term localization following intraportal transplantation: H. Fujioka, et al.; Cell Transplant.
3, 397 (1994),
Abstract;
