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5(6)-CDCFDA (ultra pure)

ROS Detector and pH indicator
ENZ-52103 100 mg 142.00 USD
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5(6)-CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. 5(6)-CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in cells with intact cell membranes. 5(6)-CFCFDA does not stain dead cells. 5(6)-CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells. Wavelength Maxima: Excitation 504nm, Emission 529nm

Product Details

Alternative Name:5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate
Purity:≥98% (HPLC)
Solubility:Soluble in DMSO.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Stable for at least one year after receipt when stored as recommended.
Handling:Protect from light. Keep cool and dry.
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Induction of ferroptotic cell death for overcoming cisplatin resistance of head and neck cancer: J.L. Roh, et al.; Cancer Lett. 381, 96 (2016), Application(s): Glutathione and ROS production measurements - cell lysates, Abstract;
Nrf2 inhibition reverses the resistance of cisplatin-resistant head and neck cancer cells to artesunate-induced ferroptosis: J. Roh, et al.; Redox Biol. 11, 254 (2016), Abstract;
Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells: M.T. Mochin, et al.; Microvasc. Res. 97, 55 (2015), Application(s): ROS detection in human endothelial cells, Abstract; Full Text
20-HETE increases superoxide production and activates NAPDH oxidase in pulmonary artery endothelial cells: M. Medhora, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008), Abstract;
The role of oxidative stress in acrolein-induced DNA damage in HepG2 cells: L. Li, et al.; Free Radic. Res. 42, 354 (2008), Abstract;

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