Replaces Prod. #: ALX-620-063
Colenterazine is useful for monitoring calcium in live cells and tissues, in reporter gene assays, for superoxide anion detection and in drug high-throughput screening applications. Coelenterazine is useful for reconstituting aequorin in cells that have been transfected with apo-aequorin cDNA. The aequorin complex comprises a 22kDa apoaequorin protein, molecular oxygen and coelenterazine. In the presence of Ca(II), coelenterazine is oxidized to coelenteramide, yielding the release of carbon dioxide and blue light. Unlike fluorescent Ca(II) indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Wavelength Maxima: Excitation 429nm, Emission 466nm
Product Details
| Alternative Name: | 2-[(4-Hydroxyphenyl)methyl]-6-(4-hydroxyphenyl)-8-(phenylmethyl)-imidazo [1,2-a] pyrazin-3-(7H)-one |
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| MW: | 423.5 |
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| CAS: | 55779-48-1 |
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| Purity: | ≥90% (HPLC) |
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| Solubility: | Soluble in 100% ethanol. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Store at -20°C, under nitrogen. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time. This material is unstable in DMSO, DMF, dimethylacetamide and N-methylpyrrolidone. Coelenterazine solution in methanol showed <10% decomposition when stored at room temperature in the dark for one month, and <2% decomposition when stored at –20°C in the dark, under nitrogen. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Characterization of coelenterazine analogs for measurements of Renilla luciferase activity in live cells and living animals: H. Zhao, et al.; Mol. Imaging
3, 43 (2004),
Abstract;
Imaging reversal of multidrug resistance in living mice with bioluminescence: MDR1 P-glycoprotein transports coelenterazine: A. Pichler, et al.; PNAS
101, 1702 (2004),
Abstract;
