5-FAM is the purified single isomer of 5,6-carboxyfluorescein and is used for labeling peptides, proteins and nucleotides through the interaction of carboxylic acid with primary amines. It has been principally used to develop a variety of green fluorescent reagents and small fluorescent molecules due to its relatively high absorbance characteristics, excellent fluorescence quantum yield, and good water solubility. The probe has also found application in studies of liposome-cell, cell-cell, and liposome-liposome interactions and in related studies on lipid bilayer structures. Wavelength Maxima: Excitation 492nm, Emission 518nm
Product Details
| Alternative Name: | 5-FAM, Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-5-carboxylic acid, 3',6'-dihydroxy-3-oxo- |
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| MW: | 376.3 |
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| CAS: | 76823-03-5 |
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| Purity: | ≥98% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Extending the applicability of carboxyfluorescein in solid-phase synthesis: R. Fischer, et al.; Bioconjug. Chem.
14, 653 (2003),
Abstract;
Semisynthesis and application of carboxyfluorescein-labelled biologically active human interleukin-8: R. David, et al.; Biol. Chem.
384, 1619 (2003),
Abstract;
