Used in a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Fluorescent calcium indicator dye developed to improve cell loading and calcium response while maintaining the convenient Fluo-3, Fluo-4 and Calcium 4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Relative to the cited dyes, FLUOFORTE® AM dye yields the brightest signal and largest assay window.
Provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
The FLUOFORTE® Reagent is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Regulatory Status:
RUO - Research Use Only
Product Literature References
UR-144, synthetic cannabinoid receptor agonist, induced cardiomyoblast toxicity mechanism comprises cytoplasmic Ca2+ and DAPK1 related autophagy and necrosis: M. Akar, et al.; Toxicol. Mech. Methods (2022), Abstract;
Dynamics of Calcium during In vitro Microspore Embryogenesis and In vivo Microspore Development in Brassica napus and Solanum melongena: A. Rivas-Sendra, et al.; Front. Plant. Sci. 8, 1177 (2017), Abstract; Full Text
Controlling fertilization and cAMP signaling in sperm by optogenetics: V. Jansen, et al.; eLife 4, e05161 (2015), Application(s): Cell Culture, Abstract; Full Text
Dual Optical Recordings for Action Potentials and Calcium Handling in Induced Pluripotent Stem Cell Models of Cardiac Arrhythmias Using Genetically Encoded Fluorescent Indicators: L. Song, et al.; Stem Cells Transl. Med. 4, 468 (2015), Abstract;
Bcl-wav and the mitochondrial calcium uniporter drive gastrula morphogenesis in zebrafish: J. Prudent, et al.; Nat. Commun. 4, 2330 (2013), Application(s): Calcium flux measurement using confocal microscopy, Abstract;
Cysteine (C)-x-C receptor 4 undergoes transportin 1-dependent nuclear localization and remains functional at the nucleus of metastatic prostate cancer cells: A.S. Don-Salu-Hewage, et al. ; PLoS One 8, e57194 (2013), Application(s): Calcium flux measurement using a microplate reader, Abstract; Full Text
In Type 1 Diabetes a Subset of Anti-Coxsackievirus B4 Antibodies Recognize Autoantigens and Induce Apoptosis of Pancreatic Beta Cells: C. Bason, et al.; PLoS One 8, e57729 (2013), Application(s): Intracellular Ca++ levels in beta cells upon Ca-channel activation, Abstract; Full Text
Late Sodium Current Inhibition Reverses Electromechanical Dysfunction in Human Hypertrophic Cardiomyopathy: R. Coppini, et al.; Circulation 127, 575 (2013), Application(s): Intracellular Ca2+ mobilization in single myocyte cell using patch-clamp, Abstract; Full Text
Activation of haem-oxidized soluble guanylyl cyclase with BAY 60-2770 in human platelets lead to overstimulation of the cyclic GMP signaling pathway: C.B. Mendes, et al.; PLoS One 7, e47223 (2012), Application(s): Calcium levels in human platelets, Abstract; Full Text
Hedgehog partial agonism drives Warburg-like metabolism in muscle and brown fat: R. Teperino, et al.; Cell 151, 414 (2012), Application(s): Calcium levels in a variety of mouse cells, Abstract;
No evidence for inositol 1,4,5-trisphosphate-dependent Ca2+ release in isolated fibers of adult mouse skeletal muscle: B. Blaauw, et al.; J. Gen. Physiol. 140, 235 (2012), Application(s): Imaging of FluoForte® fluorescence in cultured C2C12 myotubes, Abstract; Full Text
General Literature References
High throughput assay technologies for ion channel drug discovery: W. Zheng, et al.; Assay Drug Dev. Technol. 2, 543 (2004), Abstract;
Novel fluo-4 analogs for fluorescent calcium measurements: V.V. Martin, et al.; Cell Calcium 36, 509 (2004), Abstract;
Signalling specificity in GPCR-dependent Ca2+ signalling: K. Kiselyov, et al.; Cell Signal. 15, 243 (2003), Abstract;
Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41: M. do Ceu Monteiro, et al.; Cytometry 35, 302 (1999), Abstract;
Loading and localization of Fluo-3 and Fluo-3/AM calcium indicators in sinapis alba root tissue: A. Tretyn, et al.; Folia Histochem. Cytobiol. 35, 41 (1997), Abstract;
Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3: C. Perez-Terzic, et al.; Cell Calcium 21, 275 (1997), Abstract;
