Replaces Prod. #: ALX-620-006
Quin-2 demonstrates a strong affinity for calcium, resulting in a marked shift in ultraviolet absorption and fluorescence spectra versus the unbound state. Quin-2 resembles calcium chelator EGTA in its ability to bind calcium more tightly than magnesium. Quin-2 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Quin-2 has lower absorptivity and quantum yield values than Fura-2, Indo-1, Fluo-3 and Fluo-4, thus requiring higher loading concentrations. Wavelength Maxima: Excitation 353nm, Emission 495nm
Product Specification
| Alternative Name: | 2-[(2-bis-[Carboxymethyl]amino-5-methylphenoxy)-methyl]-6-methoxy-8-bis[carboxymethyl]aminoquinoline |
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| Formula: | C28H23N3O10 . 4K |
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| MW: | 693.9 |
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| CAS: | 73630-23-6 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Yellow solid. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2: J.R. Lakowicz, et al.; Cell Calcium
15, 7 (1994),
Abstract;
Fluorescence lifetime imaging of calcium using Quin-2: J.R. Lakowicz, et al.; Cell Calcium
13, 131 (1992),
Abstract;
