Replaces Prod. #: ALX-620-009
Rhod-2 AM has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, making it suitable for use with argon and krypton laser excitation sources. Rhod-2 signal intensity one of the strongest of all the calcium probes. Rhod 2 is especially suitable probe for intracellular calcium monitoring by confocal laser-scanning microscopy and flow cytometry. The dissociation constant of Rhod 2 with calcium is among the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Rhod-2 AM is an acetoxymethyl ester derivative of Rhod-2 that can be easily loaded into cells by passive incubation. Wavelength Maxima: Excitation 549nm, Emission 578nm
Product Details
| Alternative Name: | 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N',N'-tetraacetic acid, tetraacetoxy methyl ester . Br |
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| MW: | 1124.0 |
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| CAS: | 145037-81-6 |
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| Purity: | ≥90% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye: A. Wolf, et al.; Nat. Commun.
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Abstract;
Full Text
MICU1 drives glycolysis and chemoresistance in ovarian cancer: P.K. Chakraborty, et al.; Nat. Commun.
8, 14634 (2017),
Abstract;
Full Text
Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching: C. Du, et al.; Cell Calcium
29, 217 (2001),
Abstract;
Mitochondrial free calcium levels (Rhod-2 fluorescence) and ultrastructural alterations in neuronally differentiated PC12 cells during ceramide-dependent cell death: M.P. Muriel, et al.; J. Comp. Neurol.
426, 297 (2000),
Abstract;
