Fluo-3 AM is widely used in flow cytometry, confocal laserscanning microscopy and fluorescence spectroscopy. The probe has proved particularly valuable for determining GPCR function via cell-based high-throughput screening. Fluo-3 AM is a membrane permeable probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-3 becomes fluorescent in the presence of free Ca (II) . Fluo-3 is a long wavelength calcium indicator probe (488 nm excitable). The longer wavelength of absorption is convenient for minimizing photodamage in cells. Wavelength Maxima: Excitation 506nm, Emission 526nm
Product Details
| Alternative Name: | 1-[Amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxy-methyl ester |
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| MW: | 1129.8 |
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| CAS: | 121714-22-5 |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Selective Inhibition of PAR4 (Protease-Activated Receptor 4)-Mediated Platelet Activation by a Synthetic Nonanticoagulant Heparin Analog: Y.C. Lin, et al.; Arterioscler. Thromb. Vasc. Biol.
39, 694 (2019),
Application(s): measurement of platelet intracellular Ca2+ mobilization in human platelet cells ,
Abstract;
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A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux: S. Bailey & P.J. Macardle; J. Immunol. Methods
311, 220 (2006),
Abstract;
A laser scanning confocal microscopy method. Simultaneous detection of intracellular Ca2+ and apoptosis using Fluo-3 and Hoechst 33342: T. Zhang, et al.; Anal. Quant. Cytol. Histol.
22, 93 (2000),
Abstract;
