Highly resistant to photo-bleaching, concentration quenching and photoconversion
Identify modulators of secretory membrane traffic and organelle integrity
Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the ORGANELLE-ID-RGB® III detection reagent is more faithfully localized to the Golgi apparatus, with minimal staining of the endoplasmic reticulum. The red dye component of the reagent stains the endoplasmic reticulum with high fidelity and is specifically designed for use with green fluorescing probes. ORGANELLE-ID-RGB® III assay kit is validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the GA to ER in cellular secretory pathways.
Figure 1: ORGANELLE-ID-RGB® III kit staining of MDCK epithelial cells. The ER-ID® Red, GOLGI-ID® Green and Hoechst 33342 (blue) dyes highlight their respective subcellular targets with high dependability.
Figure 2: 3D Imaging of ER (red) and Golgi body (green) displayed relative to nucleus (blue). Three-dimensional reconstruction of the spatial relationship between the Golgi body and the endoplasmic reticulum using a structured illumination method. This was accomplished with the Apotome device from Carl Zeiss, Inc. This imaging hardware enabled creation of optical sections through the cell using a conventional wide field fluorescence microscope, for improved resolution along the optical axis. The optical sections were then used to create a 3-D reconstruction of the endoplasmic reticulum (red) and Golgi body (green), enabling display in their proper spatial context relative to the nucleus (blue).
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Product Details
Applications:
Fluorescence microscopy, Fluorescent detection
Application Notes:
The ORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the Golgi apparatus to endoplastic reticulum in cellular secretory pathways.
Quality Control:
A sample from each lot of ORGANELLE-ID-RGB® III assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivities of the red, green and blue stains are evident.
Quantity:
100 assays. For detection of the Golgi apparatus, endoplasmic reticulum and nucleus by microscopy.
Use/Stability:
With proper storage, the kit components are stable for 1 year from date of receipt.
Handling:
Protect from light. Avoid freeze/thaw cycles.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Contents:
ORGANELLE-ID-RGB® Reagent III (lyophilized), 1 vial 10X Assay Buffer 1, 15 ml 50X Assay Buffer 2, 1.2 ml
Technical Info/Product Notes:
The ORGANELLE-ID-RGB® III Assay Kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Oxidative-Stress-Mediated ER Stress Is Involved in Regulating Manoalide-Induced Antiproliferation in Oral Cancer Cells: S.Y. Peng, et al.; Int. J. Mol. Sci. 24, 3987 (2023), Abstract;
Newtic1 Is a Component of Globular Structures That Accumulate along the Marginal Band of Erythrocytes in the Limb Blastema of Adult Newt, Cynops pyrrhogaste: X. Chen, et al.; Biomedicines 10, 2772 (2022), Abstract;
Zn-doped CuO nanocomposites inhibit tumor growth by NF-κB pathwaycross-linked autophagy and apoptosis: H. Xu, et al.; Nanomedicine (Lond.) 14, 131 (2019), Application(s): Confocal microscopy in cancer cells (Panc28 and HepG2), Abstract;
In vitro administration of gold nanoparticles functionalized with MUC-1 protein fragment generates anticancer vaccine response via macrophage activation and polarization mechanism: T. Mocan, et al.; J. Cancer 6, 583 (2015), Application(s): Fluorescence microscopy, Abstract; Full Text
Photothermal treatment of liver cancer with albumin-conjugated gold nanoparticles initiates Golgi Apparatus-ER dysfunction and caspase-3 apoptotic pathway activation by selective targeting of Gp60 receptor: L. Mocan, et al.; Int. J. Nanomedicine 10, 5435 (2015), Application(s): Fluorescence microscopy, Abstract; Full Text
General Literature References
The Golgi apparatus as a crossroads in intracellular traffic: A. Mironov & M. Pavelka; The Golgi Apparatus: State of the art 110 years after Camillo Golgis Discovery Mironov and Pavelka, Eds., Springer, New York, (2008), Book, Abstract;
Golgi-disturbing agents: A. Dinter & E.G. Berger; Histochem. Cell. Biol. 109, 571 (1998), Abstract;
Fluorescent conjugates of brefeldin A selectively stain the endoplasmic reticulum and Golgi complex of living cells: Y. Deng, et al.; J. Histochem. Cytochem. 43, 907 (1995), Abstract;
