The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye. Relative to analogous turbidimetric assays of PDI activity, the fluorescence-based assay provides a vastly improved assay signal window, improved lower detection limit, and superior Z’-score (>0.8). Intra-plate and inter-plate CVs using the assay are typically 3-5%. Human recombinant PDI enzyme (EC 5.3.4.1) and the PDI inhibitor, bacitracin, are included in the kit, as well as all reagents necessary for monitoring changes in PDI activity. The PROTEOSTAT® PDI assay kit is capable of providing a quantitative readout of PDI enzymatic activity in a robust and high-throughput fashion and can be applied to identification of PDI inhibitors from chemical libraries.
Product Specification
| Alternative Name: | Protein disulfide isomerase |
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| Applications: | Fluorescent detection
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| Application Notes: | This kit provides a simple, homogenous assay for screening modulators of protein disulfide isomerase (PDI) enzymatic activity. |
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| Quality Control: | Using the procedure described in the manual, a sample PROTEOSTAT® PDI assay kit is used to assay the following: (1) Insulin with PDI; (2) Insulin without PDI; and (3) Insulin with PDI and bacitracin. The Z’-factor is greater than 0.8. PDI is inhibited by at least 50% with bacitracin. |
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| Use/Stability: | With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20˚C in a non-frost free freezer, or –80˚C for longer term storage. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | PROTEOSTAT® PDI detection reagent
PDI (human) (recombinant)
Insulin (Lyophilized)
Bacitracin (Inhibitor Control, Lyophilized)
PBE buffer
Stop reagent
DTT (10mM)
Deionized water |
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| Technical Info/Product Notes: | Enzo and PROTEOSTAT® are trademarks of Enzo Life Sciences, Inc. Several of Enzo's products and product applications are covered by US and foreign patents and patents pending. |
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| UniProt ID: | P07237 (human) |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
PDI knockdown inhibits seizure activity in acute seizure and chronic epilepsy rat models via S-nitrosylation-independent thiolation on NMDA receptor: A.R. Jeon, et al.; Front. Cell. Neurosci.
12, 438 (2018),
Application(s): Hippocampal tissue lysates,
Abstract;
Full Text
The thiosemicarbazone Me2NNMe2 induces paraptosis by disrupting the ER thiol redox homeostasis based on protein disulfide isomerase inhibition: S. Hager, et al.; Cell Death Dis.
9, 1052 (2018),
Abstract;
Full Text
Tissue Factor Coagulant Activity is Regulated by the Plasma Membrane Microenvironment: Y. Yu, et al.; Thromb. Haemost.
118, 990 (2018),
Application(s): In-vitro activity assay,
Abstract;
Curcumin and Curcuma longa L. extract ameliorate lipid accumulation through the regulation of the endoplasmic reticulum redox and ER stress: H.Y. Lee, et al.; Sci. Rep.
7, 6513 (2017),
Application(s): PDI assay of tissue lysates,
Abstract;
Full Text
Nox4 regulates the eNOS uncoupling process in aging endothelial cells: H.Y. Lee, et al.; Free Radic. Biol. Med.
113, 26 (2017),
Application(s): PDI assay of HUVEC cell lysates,
Abstract;
PDI regulates seizure activity via NMDA receptor redox in rats: J.Y. Kim, et al.; Sci. Rep.
7, 42491 (2017),
Application(s): Hippocampal tissue lysates,
Abstract;
Full Text
Protein disulfide isomerase regulates renal AT1 receptor function and blood pressure in rats: X. Wang, et al.; Am. J. Physiol. Renal. Physiol.
313, F461 (2017),
Application(s): Renal tissue lysates,
Abstract;
Full Text
Requirement of zinc transporter SLC39A7/ZIP7 for dermal development to fine-tune endoplasmic reticulum function by regulating protein disulfide isomerase: B.H. Bin, et al.; J. Invest. Dermatol.
137, 1682 (2017),
Application(s): ER fractions from murine osteoblasts,
Abstract;
Succination of protein disulfide isomerase links mitochondrial stress and endoplasmic reticulum stress in the adipocyte during diabetes: A.M. Manuel, et al.; Antioxid. Redox Signal.
27, 1281 (2017),
Application(s): 3T3-L1 adipocytes and adipose tissue lysates,
Abstract;
Full Text
Adverse outcomes associated with cigarette smoke radicals related to damage to protein disulfide isomerase: H. Kenche, et al.; J. Biol. Chem.
291, 4763 (2016),
Abstract;
Adverse Outcomes Associated with Cigarette Smoke Radicals Related to Damage to Protein-disulfide Isomerase: H. Kenche, et al.; J. Biol. Chem.
291, 4763 (2016),
Application(s): in-vitro assay with recombinant PDI,
Abstract;
Full Text
Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity: K. Mätlik, et al.; Cell Death Dis.
6, e2032 (2015),
Abstract;
Full Text
Methods of measuring protein disulfide isomerase activity: a critical overview: M.M. Watanabe, et al.; Front. Chem.
2, 73 (2014),
Application(s): Review on available assays to measure PDI activity,
Abstract;
Full Text
N-octanoyl dopamine treatment of endothelial cells induces the unfolded protein response and results in hypometabolism and tolerance to hypothermia: E. Stamellou, et al.; PLoS One
9, e99298 (2014),
Abstract;
Full Text
The protein disulfide isomerases PDIA4 and PDIA6 mediate resistance to cisplatin-induced cell death in lung adenocarcinoma: G. Tufo, et al.; Cell Death Differ.
21, 685 (2014),
Application(s): Evaluation of PDI activity in whole extracts and ER fractions,
Abstract;
Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase: F. Langer, et al.; Blood
121, 2324 (2013),
Abstract;
Oxidative processing of latent Fas in the endoplasmic reticulum controls the strength of apoptosis: V. Anathy, et al.; Mol. Cell. Biol.
32, 3464 (2012),
Abstract;
General Literature References
Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase: S. Abromaitis & R.S. Stephens; PLoS Pathog.
5, e1000357 (2009),
Abstract;
Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages: C. X. Santos, et al.; J. Leukoc. Biol.
86, 989 (2009),
Abstract;
The proteostasis boundary in misfolding diseases of membrane traffic: D.M. Hutt, et al.; FEBS Lett.
583, 2639 (2009),
Abstract;
Increasing melanoma cell death using inhibitors of protein disulfide isomerases to abrogate survival responses to endoplasmic reticulum stress: P.E. Lovat, et al.; Cancer Res.
68, 5363 (2008),
Abstract;
Targeting homeostatic mechanisms of endoplasmic reticulum stress to increase susceptibility of cancer cells to fenretinide-induced apoptosis: the role of stress proteins ERdj5 and ERp57: M. Corazzari, et al.; Br. J. Cancer
96, 1062 (2007),
Abstract;
Coupling endoplasmic reticulum stress to the cell death program: R.V. Rao, et al.; Cell Death Differ.
11, 372 (2004),
Abstract;
Protein-disulfide isomerase-mediated reduction of two disulfide bonds of HIV envelope glycoprotein 120 occurs post-CXCR4 binding and is required for fusion: R. Barbouche, et al.; J. Biol. Chem.
278, 3131 (2003),
Abstract;
Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells: C.H. Davis, et al.; Infect. Immun.
70, 3413 (2002),
Abstract;
Protein-disulfide isomerase-mediated reduction of the A subunit of cholera toxin in a human intestinal cell line: P.A. Orlandi; J. Biol. Chem.
272, 4591 (1997),
Abstract;
Acceleration of reactivation of reduced bovine pancreatic ribonuclease by a microsomal system from rat liver: R.F. Goldberger, et al.; J. Biol. Chem.
238, 628 (1963),
Abstract;
