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Directly monitors global levels of superoxide in live cells by fluorescence microscopy or flow cytometry
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High sensitivity, specificity and accuracy for live cell studies
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Compatible with major components of tissue culture media (phenol red, FBS and BSA)
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Complete set of reagents, including ROS inducer and scavenger
Enzo Life Sciences’ ROS-ID® Superoxide detection kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with a standard orange (e.g., 550/620 nm) fluorescent cube, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. The Superoxide Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension).
Product Details
| Applications: | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
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| Application Notes: | This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. |
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| Quality Control: | A sample from each lot of ROS-ID® Superoxide detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard orange (e.g., 550/620 nm) fluorescent cube.
The following results are obtained: ROS positive control samples induced with Pyocyanin exhibit bright orange fluorescence in the nucleus. Cells pretreated with the ROS inhibitor don’t demonstrate any orange fluorescence signal upon induction. |
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| Quantity: | 200 fluorescence microscopy assays or 50 flow cytometry assays. |
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| Use/Stability: | With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Contents: | Superoxide Detection Reagent (Orange), 300 nmoles
ROS Inducer (Pyocyanin), 1 µmole
ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg
Wash Buffer Salts, 1 pack |
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| Technical Info/Product Notes: | The ROS-ID® Superoxide detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Rational design of mitochondria targeted thiabendazole-based Ir (III) biscyclometalated complexes for a multimodal photodynamic therapy of cancer: I. Echevarria, et al.; J. Inorg. Biochem.
231, 111790 (2022),
Abstract;
A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration: K. Bijnens, et al.; Biomolecules
11, 714 (2021),
Abstract;
Full Text
An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination: Y. Wang, et al.; Nat. Commun.
12, 4964 (2021),
Abstract;
Assay for advanced glycation end products generating intracellular oxidative stress through binding to its receptor: T. Kobori, et al.; Anal. Biochem.
611, 114018 (2020),
Abstract;
Epithelial cadherin regulates transition between the naïve and primed pluripotent states in mouse embryonic stem cells: A.M. Sharaireh, et al.; Stem Cells
38, 1292 (2020),
Abstract;
Full Text
Loss of Spry1 reduces growth of BRAFV600-mutant cutaneous melanoma and improves response to targeted therapy: B. Montico, et al.; Cell Death Dis.
11, 392 (2020),
Abstract;
Full Text
PCSK9 expression in the ischemic heart and its relationship to infarct size, cardiac function and development of autophagy: Z. Ding, et al.; Cardiovasc. Res.
114, 1738 (2018),
Abstract;
Full Text
SPARC paucity alleviates superoxide-mediated oxidative stress, apoptosis, and autophagy in diabetogenic hepatocytes: K.R. Aseer, et al.; Free Radic. Biol. Med.
108, 874 (2017),
Application(s): Fluorescence microplate reader using rat hepatocytes,
Abstract;
Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression: L. Pasterk, et al.; Sci. Rep.
6, 22104 (2016),
Application(s): Intracellular ROS production,
Abstract;
TRPC6 channel activation promotes neonatal glomerular mesangial cell apoptosis via calcineurin/NFAT and FasL/Fas signaling pathways: H. Soni, et al.; Sci. Rep.
6, 29041 (2016),
Application(s): Superoxide generation and ROS determination,
Abstract;
Full Text
Hemodynamic Shear Stress via ROS Modulates PCSK9 Expression in Human Vascular Endothelial and Smooth Muscle Cells and Along the Mouse Aorta: Z. Ding, et al.; Antioxid. Redox Signal.
22, 760 (2015),
Application(s): Flow Cytometry,
Abstract;
Full Text
Influenza A virus PB1-F2 is involved in regulation of cellular redox state in alveolar epithelial cells: N. Shin, et al.; Biochem. Biophys. Res. Commun.
459, 699 (2015),
Application(s): Flow Cytometry,
Abstract;
General Literature References
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects: P. Wardman; Free Radic. Biol. Med.
43, 995 (2007),
Abstract;
Fluorescence probes used for detection of reactive oxygen species: A. Gomes, et al.; J. Biochem. Biophys. Methods
65, 45 (2005),
Abstract;
Determination of mitochondrial reactive oxygen species: methodological aspects: C. Batandier, et al.; J. Cell. Mol. Med.
6, 175 (2002),
Abstract;
Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite: M.M. Tarpey & I. Fridovich; Circ. Res.
89, 224 (2001),
Abstract;
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