Red 594 dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 594 dUTP can also be used for multicolor fluorescence labelling.
Product Specification
| Concentration: | 1mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 594 nm |
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| Emission maximum: | 614 nm |
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| Extinction Coefficient: | 115,000 M-1cm-1 (594 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA] |
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| Purity: | ≥85% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Appearance: | Red solution. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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