Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Aqua431dUTP can also be used for multicolor fluorescence labeling.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
Alternative Name:
DEAC dUTP
Quantity:
Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
Concentration:
1mM
Formulation:
Liquid. Solution in water.
Excitation maximum:
431 nm
Emission maximum:
480 nm
Extinction Coefficient:
50,000 M-1 cm-1 (431 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
Correction Factor (260 nm):
0.14
Correction Factor (280 nm):
0.14
Purity:
≥93% (HPLC)
Purity Detail:
Purified by ion-exchange chromatography.
Appearance:
Yellow liquid.
Applications:
FISH
Shipping:
Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
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Product Literature References
Highly Multiplexed Fluorescence in Situ Hybridization for in Situ Genomics: M.L. Onozato, et al.; J. Mol. Diagn. 21, 390 (2019), Application(s): Multiplex 2 and 3 colors in combination with Green 496-dUTP, Gold 525-dUTP, Orange 552-dUTP, Red 580-dUTP, Red 650 (Cy5)-dUTP, Abstract; Full Text
Development of a database system and image viewer to assist in the correlation of histopathologic features and digital image analysis with clinical and molecular genetic information: Y. Yagi, et al.; Pathol. Int. 66, 63 (2016), Application(s): 3-color FISH (Green 496 dUTP, Orange 552 dUTP, Aqua 431dUTP labeled probes), Abstract;
