Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures.
Product Details
| Alternative Name: | Fluorescein-12-uridine-5’-triphosphate |
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| Formula: | C39H41N4O22P3 |
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| MW: | 1010.7 (free acid) |
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| Concentration: | 10mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 496 nm |
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| Emission maximum: | 520 nm |
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| Extinction Coefficient: | 85,000 M-1 cm-1 (496 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Appearance: | Yellow-green liquid and orange frozen solution. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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