Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer slectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The biotin-labeled hybridized probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate.
Product Details
Alternative Name: | Biotin-16-uridine-5’-triphosphate |
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Formula: | C32H52N7O19P3S |
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MW: | 963.7 (free acid) |
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Concentration: | 10mM |
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Formulation: | Liquid. Solution in water. |
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Extinction Coefficient: | 10,700 M-1 cm-1 (240 nm, pH 7.0) |
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Purity: | ≥93% (HPLC) |
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Purity Detail: | Contains <5% Bio-16-UDP. |
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Appearance: | Clear, colorless liquid. |
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Shipping: | Dry Ice |
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Long Term Storage: | -20°C |
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Handling: | Avoid freeze/thaw cycles. |
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Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Whole-genome transcription and DNA methylation analysis of peripheral blood mononuclear cells identified aberrant gene regulation pathways in systemic lupus erythematosus: H. Zhu, et al.; Arthritis Res. Ther.
18, 162 (2016),
Application(s): Gene expression studies, RNA samples,
Abstract;
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