For preparation of Cyanine 3- and Cyanine 5-labeled DNA for hybridization to BAC arrays.The CGH Labeling Kit for BAC Arrays has been optimized to label DNA for dual sample comparative genomic hybridization (CGH) assays on BAC arrays. All required reagents are provided including fully optimized nucleotide mixes that contain either cyanine 3-dUTP or cyanine 5-dUTP.The CGH Labeling Kit for BAC Arrays has been specifically tailored to meet the varying requirements of individual laboratories by providing sufficient amount of reagents for two different protocols. For the average user, the Standard Protocol calls for a 4-hour labeling in a 50µl reaction volume. For users with dilute DNA (≤ 20 ng/µl), the Dilute DNA Protocol calls for a 4-16 hour labeling in a total volume of 100µl.
Product Details
| Applications: | CGH
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| Quantity: | Contains reagents sufficient for 20 reactions or 10 reactions in duplicate with a dye swap. |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Contents: | Primers/Reaction Buffer, 2 x 400 µl
Cyanine 3-dUTP Nucleotide Mix, 2 x 50 µl
Cyanine 5-dUTP Nucleotide Mix, 2 x 50 µl
Klenow Exo-DNA Polymerase, 1 x 20 µl
Stop Buffer, 1 x 100 µl
Nuclease-free Water, 1 x 1 ml |
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| Technical Info/Product Notes: | For additional information on the CGH Labeling Kit for BAC Arrays please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Platform comparisons for identification of breast cancers with a BRCA-like copy number profile: P.C. Schouten, et al.; Breast Cancer Res.
139, 317 (2013),
Application(s): Breast cancer,
Abstract;
Molecular karyotypes of Hodgkin and Reed-Sternberg cells at disease onset reveal distinct copy number alterations in chemosensitive versus refractory Hodgkin lymphoma: M.L. Slovak, et al.; Clin. Cancer Res.
17, 3443 (2011),
Abstract;
Full Text
Gab2-mediated signaling promotes melanoma metastasis: B. Horst, et al.; Am. J. Pathol.
174, 1524 (2009),
Application(s): Metastatic melanoma,
Abstract;
Full Text
