BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System

Lower RNA Input with High Yield – High Quality Biotin-labeling



ENZ-42422-0010	10 Reactions	975.00 USD
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Single tube reaction for amplification and labeling
Generates sufficient aRNA for standard microarray analysis from as little as 20 ng of total input RNA
Superior 3’/5’ transcript ratios demonstrating efficient in vitro transcription
3’/5’ ratios significantly better, as Enzo kit completes transcript through the 5’ end

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis. This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 20 ng and up to 500 ng. Entire amplification reaction can be performed in a single tube and the biotin-labeled aRNA can be purified using either magnetic beads or purification columns. The BIOARRAY™ Low Input aRNA Amplification and Biotin Labeling System has been optimized for superior performance with reduced variability and improved reproducibility and data quality. The complete system is composed of reagents for cDNA synthesis and in vitro transcript labeling.

BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System Web1

Figure 1: Quality assessment of aRNA obtained using BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System. The 3’/5’ ratios, on an Affymetrix U133a array, for housekeeping gene Actin obtained using Enzo kit are much lower than the ones from the competitor’s indicating higher quality of aRNA obtained using Enzo kit.

BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System Web2

Figure 2: Universal Human Reference RNA ranging from 20 ng to 500 ng was amplified in triplicate using BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System. The lowest amount of input (20 ng) generated enough labeled aRNA for Microarray Analysis.

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Product Details

Application Notes:	The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for gene expression analysis.

Quality Control:	aRNA yield must ≥ 20µg aRNA purity must range between an A₂₆₀/A₂₈₀ ratio of 1.9 and 2.3 aMedian aRNA size must be equal to or greater than 1200 nt Affymetrix GeneChip criteria: % Present calls ≥ 40% Scale factors ≤ 3.0 3'/5' ratios for actin and GAPDH ~ 3.0 and 1.5 respectively

Use/Stability:	Store at -20˚C in a non-frost free freezer.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Dry Ice

Long Term Storage:	-20°C

Contents:	40 µl, First Strand Buffer (FSB) 10 µl, First Strand Enzyme Mix (FSE) 30 µl, Second Strand Buffer Mix (SSB) 20 µl, Second Strand Enzyme Mix (SSE) 250 µl, Transcription Buffer Mix (TB) 75 µl, DTT (DTT) 75 µl, Enhancer Cocktail (EC) 50 µl, T7 RNA Polymerase (T7) 1 ml, Nuclease-free Water (W)

Technical Info/Product Notes:	This product is manufactured and sold by ENZO LIFE SCIENCES, INC. for research use only by the end-user in the research market and is not intended for diagnostic or therapeutic use. Purchase does not include any right or license to use, develop or otherwise exploit this product commercially. Any commercial use, development or exploitation of this product or development using this product without the express prior written authorization of ENZO LIFE SCIENCES, INC. is strictly prohibited. Enzo and BIOARRAY are trademarks of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

Regulatory Status:	RUO - Research Use Only

Product Literature References

HELLS Is Negatively Regulated by Wild-Type P53 in Liver Cancer by a Mechanism Involving P21 and FOXM3: S. Schuller, et al.; Cancers 14, 459 (2022), Abstract;

General Literature References

Amplified RNA synthesized from limited quantities of heterogeneous cDNA: R.N. Van Gelder, et al.; PNAS USA 87, 1663 (1990), Abstract;

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