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BIO-PROBE® SV40 probe

 
ENZ-40845 2 µg 287.00 USD
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The SV40 BIO-PROBE® labeled probe is prepared by nick translation of the entire genome of SV40, a member of the papovavirus family. SV40 has served as a model virus for studies of transformation and gene regulation. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Product Specification

Concentration:20µg/ml
 
Formulation:Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
 
Applications:Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
 
Application Notes:The SV40 BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at a concentration of approximately 50ng/ml. The probe can also be used for in situ hybridization applications at concentrations of approximately 0.5 to 2.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes.Complete kits and protocols are available for in situ hybridization and detection procedures (Enzo’s SAVIEW® PLUS and POLYVIEW® PLUS in situ detection systems).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for one year after receipt when stored as recommended.
 
Technical Info/Product Notes:BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Molecular identification of SV40 infection in human subjects and possible association with kidney disease: R.M. Li, et al.; J. Am. Soc. Nephrol. 13, 2320 (2002), Abstract;
Progressive multifocal leukoencephalopathy and oligodendroglioma in a monkey co-infected by simian immunodeficiency virus and simian virus 40: F. Chretien, et al.; Acta Neuropathol. 100, 332 (2000), Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;

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