The BIO-PROBE® Epstein-Barr virus (EBV) labeled probe is prepared by nick translation of the 3Kb Bam HI "W" region (the internal repeat) of the EBV genome. The Bam HI clone is contained in pUC 18 vector. The probe does not cross hybridize with the other herpes virus DNAs, herpes simplex virus DNA or cytomegalovirus DNA.
Product Details
Concentration:
20 µg/mL
Formulation:
Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
Applications:
Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
Application Notes:
The BIO-PROBE® Epstein-Barr virus (EBV) probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100 ng/mL. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 2.0 µg/mL. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin, or antibodies coupled to fluorescent dyes or color producing enzymes.
Shipping:
Dry Ice
Long Term Storage:
-20°C
Technical Info/Product Notes:
BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo's products and product applications are covered by US and foreign patents and patents pending.
